Cas3
Cas3 is an ATP-dependent single-strand DNA (ssDNA) translocase/helicase enzyme that degrades DNA as part of CRISPR based immunity.[1]
| CRISPR-associated protein 3 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | cas3 | ||||||||
| Pfam | PF18395 | ||||||||
| InterPro | IPR041372 | ||||||||
| |||||||||
Cas3 is a "signature" protein of class 1 CRISPR systems and functions in a complex known as CASCADE, with other cas genes and a targeting RNA to degrade viral DNA.[1]
In April 2019 Cornell University researcher Ailong Ke published a paper in the journal Molecular Cell describing a new gene editing CRISPR system, CRISPR-Cas3 which can efficiently delete long swaths of DNA from a targeted site in the human genome. This ability is superior to that achieved with the more common CRISPR-Cas9 systems.[2]
CONAN, a CRISPR based diagnostic approach was developed utilising Cas3 [3]
References
- He L, St John James M, Radovcic M, Ivancic-Bace I, Bolt EL (February 2020). "Cas3 Protein-A Review of a Multi-Tasking Machine". Genes. 11 (2): 208. doi:10.3390/genes11020208. PMC 7074321. PMID 32085454.
- "CRISPR-Cas3 innovation holds promise for disease cures, advancing science". Cornell Chronicle. Retrieved 2020-09-07.
- Yoshimi, Kazuto; Takeshita, Kohei; Yamayoshi, Seiya; Shibumura, Satomi; Yamauchi, Yuko; Yamamoto, Masaki; Yotsuyanagi, Hiroshi; Kawaoka, Yoshihiro; Mashimo, Tomoji (February 2022). "CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus". iScience. 25 (2): 103830. Bibcode:2022iSci...25j3830Y. doi:10.1016/j.isci.2022.103830. ISSN 2589-0042. PMC 8801231. PMID 35128347.
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