Helix (gastropod)

In Linnaeus' 10^th edition of Systema Naturae, which marks the beginning of the zoological nomenclature, the generic name Helix had been used for a variety of terrestrial (e.g. Zonites algirus), freshwater (e.g. Lymnaea stagnalis), and marine (e.g. Fossarus ambiguus) gastropods; later this was restricted to stylommatophoran species with flattened to globular shells, including zonitids and other groups. In the course of the 1800s, more groups were removed, but prior to 1900, several thousand helicid and hygromiid species of Europe and abroad had still been classified in the genus Helix.[8][9] By the early 1900s, the genus was split into many separate genera, leaving only some 30 species closely related to its type species Helix pomatia in the genus. However, due to the previously broad concept of the genus, Helix is part of the original combination (basionym) of many gastropod names and there still are many nominal taxa described in Helix whose generic placement remains unresolved (taxa inquirenda),[10] although they clearly do not refer to any species of Helix in its present sense.

In the 2000s, Helix has been subject to extensive molecular phylogenetic studies and taxonomic revisions.[1][11][2][12][13] These led to the exclusion of several species, most notably the garden snail, and inclusion of others (H. ceratina, H. nicaeensis). Maltzanella, for long considered a subgenus of Helix, is the sister group of Helix.

Two subgenera are currently recognized:[2]

• Helix (Helix) Linnaeus, 1758
• Helix (Pelasga) P. Hesse, 1908

Helix comprises large land snails species, with shell diameter of 2-6 cm. The shell is globular to conical, with five darker bands that may be reduced or fuse together. The globular shell distinguishes Helix from most of the related genera (tribe Helicini), except for Maltzanella and Lindholmia. The surface has a structure of fine transversal ribs, developed to a varying degree, and there may be very fine spiral grooves as well. The shell is never malleated. Colour of the foot varies. It may be grey, brown, black or pink; the back of the foot is dark in several species.

Characters on the genital system have been used to define the genus and its subgenera. Unlike Cornu, the penis of Helix contains two papillae with a central opening. There appears to be a tendency for a shortening of the diverticulum of bursa copulatrix and of the eppiphallus, but there is an overlap with related genera in these characters. Mucous glands adjoining the dart sac are ususally richly branched.

Helix is a western Palaearctic genus. The species diversity is concentrated to the Balkans and Anatolia, with the greatest phylogenetic diversity in Greece. The natural western distribution limits run through mainland France (Helix pomatia), Corsica (Helix ceratina), and Algeria (Helix melanostoma). In the north, the natural distribution of H. pomatia reaches central Germany and the southern margins of the North European plain. The southernmost species live in North Africa (H. melanostoma, H. pronuba) and the southern Levant (H. engaddensis). The eastern limits are reached in western Iran and Iraqi Kurdistan (H. salomonica) and in the Caucasus (H. lucorum); H. thessalica reaches through Ukraine at least to the western Russian frontier.[1][2]

The haploid number of chromosomes is 27 (studied species were H. lucorum, H. buchii, H. pomatia, H. gussoneana and H. straminea).[14][15][16][17] In H. pomatia, all chromosomes have median or sub-median centromeres.[18] Small supernumerary chromosomes were reported from H. pomatia from England.[18]

Haploid genome size was estimated to be nearly 4 Gbp (C-value 4 pg) with a GC-content of ~42%,[19][20] but its is unclear which species was studied due to a discrepancy between the stated species and sample origin.

The structure of the genital system corresponds in most aspects to that of other Helicidae. Its anatomy and function has been studied in detail in H. pomatia.[21][22][23]

As all stylommatophorans, Helix snails are hermaphrodites. Sperms and egg cells are produced in a common gonad, the ovotestis (hermaphroditic gland), which is embedded in the hepatopancreas (digestive gland) near the apex of the shell. Gametes are transported through a hermaphroditic duct (ovotestis duct) to the fertilization pouch–spermatheca complex (carrefour) embedded at the base of the albumen gland.[24] In this organ, the foreign sperm is stored in spermathecal sacs (receptacula seminis) and egg fertilization takes place in the fertilisation pouch. The albumen gland provides the provision of the developing fertilized eggs, and its size greatly varies with the stage of the reproductive cycle. Own sperm and fertilized eggs are transported by specialised regions of the spermoviduct (sperm groove and uterus), which distally separate into a male (vas deferens) and female (free oviduct) parts of the genital system.[24]

The male genitalia consist of a tube that serves the formation of a spermatophore and its transfer into the female parts of the mating partner. Penis is the most distal and muscular part. A spermatophore is built in the epiphallus (between vas deferens and penis) and the flagellum (continuation of the epiphallus proximally from the vas deferens opening); flagellum (the latter forms the tail of the spermatophore. During copulation, the penis everts (like a sleeve turned inside out)[21] and is in this process inserted in the vagina. A retractor muscle attaches on the epiphallus.

The female part consists of a vagina, the dart apparatus that opens into it and the bursa copulatrix (gametolytic gland). Vagina serves the transport of the foreign spermatophore and of eggs. The bursa is attached by a thin stalk to the vagina (marking the boundary between vagina and the free oviduct). The stalk in most cases bears a diverticulum, a blind tube that receives the front part of the spermatophore if present. The diverticulum has been proposed to be a remnant of a seminal duct that originally transported foreign sperm (sperm from the other individual) into the fertilization pouch.[25] Sperm leave the tail of the spermatophore and migrate into the oviduct and then to the fertilization pouch; vast majority of the sperm does not escape in this way and is digested in the bursa.[23][24] In Helix, there is tendency for a reduction of the diverticulum, and it can be missing in several species.[2]

The dart apparatus is composed of a muscular dart sac and two mucous glands (digitiform glands) on its sides. The mucous glands are banched at the base; the number of branches varies between Helix species. The dart is aragonitic, straight or only weakly curved, with four blades (vanes) along its length and a corona at its base.[26][5] Dart apparatus is missing in Helix salomonica.[2]

The male and female parts open into a common atrium and a genital pore positioned ventrally behind the right optic tentacle.

Helix pomatia in the initial phase of the mating sequence.

The aspects of reproduction have been studied primarily in H. pomatia, with limited information from other species.

Mating behaviour has been described several times for H. pomatia.[21][27] In the initial phase, the two snails raise their foots and press the soles against each other and touch each others tentacles and mouthparts. This takes 15–30 min. Some time later, the dart shooting takes place, although many matings progress without a love dart being employed. The mucous glands produce produce a whittish secret just before the shooting, that contains hormones promoting the compound that improves preservation of foreign sperm in the receiving individual.[28] Then, again after a pause, comes the copulation, usually preceeded by several unsuccessful attempts in which the reciprocal insertion of penes is not achieved and the genital organs are partially retracted back into the body. Finally, both individuals simultaneously insert the penes into each others female opening. Within ca. 4–7 min the spermatophore is formed and transferred, after which the snail disengage and retract the everted genital organs. However, the complete reception of the spermatophore takes another 2-3 hours, during which the snails remain partially retracted and inactive.[29][30]

It has been reported that only one spermatophore is usually transferred during copulation in H. pomatia, so one animal functions as a male and one as a female in each mating. According to that report it is mostly the older snail who lay eggs, while younger function as males.[29]

Received foreign sperm may be stored for more than a year before fertilization.[23]

In H. pomatia, eggs are laid into a chamber digged in the soil by the parent 4–6 days after mating.[29] Eggs are formed only as the nest is built.[31] As in other pulmonates, the eggs are rich in galactogen produced by the albumen gland. The eggshell is partially mineralized, with crystals of calcium cabonate in a flexible membrane.[24] Hatching follows roughly 25–26 days after egg laying, but the snails remain additional 8–10 days in the nest.[29]

From April through the northern summer, the number of snails copulating increases due to the higher temperature and humidity, which enhance the possibility of oviposition. The pulmonate snails are hermaphroditic, meaning that both female and male sexual organs are present in the same individual. The snails produce both eggs and sperm in the ovotestis (also called the hermaphrodite gland), but it is later separated into two divisions, a sperm duct and oviduct, respectively.

In H. pomatia, sexual maturity is typically reached after 2-3 overwinterings, i.e. at the age of 2-4 years.[29] Some individuals may live longer, perhaps even 30 years or older in the case of the H. pomatia[32] but most live less than 8 years. Many deaths are due to predators and parasites.

Species of Helix live in a variety of habitats and under very different climatic regimes. Some species are exclusively found in open limestone rocky habitats (H. secernenda), while others tolerate acidic bedrock (e.g. H. pelagonesica) or live predominantly in forests (e.g. H. thessalica). Members of the genus occur from temperate rainforests (H. buchii) to semi-arid regions.

Some bird species, like corvids, prey on Helix species.

The kinetoplastid Cryptobia helicis lives in the bursa copulatrix of Helix pomatia.[23]

Some species, above all H. pomatia and H. lucorum, are being collected or farmed for human consumption. The culinary use dates back several millenia and has been evidenced for several species across the genus' range. Mesolithic shell midden dated to 9370±80 and 8110±90 uncalibrated C-14 years bp and providing evidence of collecting was documented for H. pomatella in Abruzzo, Italy.[33]

Most of the species included in the IUCN Red List are classified as Least Concern.[34] One of the species, H. ceratina, is critically endangered and the present known distribution is limited to a very small area near the Ajaccio airport.[35]

In the past, collection of wild H. pomatia for food led to fears of over-exploitation and the introduction of protection by law in several countries.[36]

Several extinct species of Helix have been described (the list is not complete):

• Helix jasonis Mayer, 1856 (Ukraine: Sevastopol. Miocene: Tortonian[39])
• Helix pseudoligata Sinzov, 1897 (Ukraine. Miocene: Sarmatian[39])
• Helix toulai Kojumdgieva, 1969 (Bulgaria: Balchik. Miocene: Sarmatian)
• Helix barbeyana De Stefani in De Stefani et al., 1891
• Helix krejcii Wenz in Krejci-Graf & Wenz, 1926
• Helix mrazeci Sevastos, 1922
• Helix sublutescens Wenz in Krejci & Wenz, 1926

Some extant species are known from Quaternary deposits. The most studied species in this respect is H. pomatia, where the fossils have been used to document the earliest postglacial occurrences in Central Europe.[40] The earliest record in Czechia was dated directly by radiocarbon to 10120-9690 BP (but is likely a few hundred years younger)[41]; fossils presumably older than 9402–9027 BP[42] or 9403–9003 BP[43] were found in Baden-Würtemberg, Germany.

The phylogenetic relationships between Helix and related genera as well as the internal relationships within the genus have been so far studied only using partial sequences of mitochondrial genes and of the nuclear rRNA gene cluster.[44][1]

The cladogram shown is based on phylogenetic analyses of mitochondrial sequence data.[45][11][37][1]

The following genus-level taxa are considered synonyms of Helix:

• Callunea Scudder, 1882
• Cochlea Da Costa, 1778
• Coenatoria Held, 1838
• Cunula Pallary, 1936
• Glischrus S. Studer, 1820
• Helicites W. Martin, 1809 (Established for fossils of Helix to distinguish them from extant members of that taxon. Invalid, available only for the purposes of the Principle of Homonymy (Art. 20))
• Helicogena A. Férussac, 1821
• Megastoma Scudder, 1882
• Naegelea P. Hesse, 1918
• Pachyphallus P. Hesse, 1918
• Pentataenia A. Schmidt, 1855 (junior objective synonym)
• Physospira Boettger, 1914
• Pomatia Beck, 1837
• Pomatiana Fagot, 1903
• Pomatiella Pallary, 1909
• Pseudofigulina P. Hesse, 1917
• Rhododerma P. Hesse, 1918
• Tacheopsis Boettger, 1909
• Tammouzia Pallary, 1939